mouse igg1 constant region  (InvivoGen)

Journal: Journal of Neuroinflammation

Article Title: Maternal immunoglobulin G affects brain development of mouse offspring

doi: 10.1186/s12974-024-03100-z

Figure Lengend Snippet: Comprehensive analysis of microglial genes differentially expressed by IgG stimulation. ( A, B ) Primary cultured microglia were immunolabeled with anti-mouse IgG antibody (green) and anti-CD16/32 antibody (red) without IgG addition ( A ) and 24 h after IgG addition to the culture media ( B ). Scale bars, 50 μm. ( C ) Volcano plots of DEGs between the control and primary cultured microglia stimulated with 10 µg/ml IgG for 24 h. Representative IFN-I-related genes are shown in red. ( D ) Graphical summary of the pathways regulated after IgG treatment generated by ingenuity pathway analysis (IPA). Orange and blue indicate activation and inhibition, respectively. The solid line indicates a direct interaction, the dashed line indicates an indirect interaction, and the dotted line indicates an inferred correlation from machine-based learning. ( E ) Significant pathways identified by IPA. Positive and negative z-scores, colored in orange and blue, represent the activation and inhibition of pathways, respectively. ( F ) Significant activation of the IFN-I signaling pathway was predicted using IPA. Orange indicates activation

Article Snippet: For microglial stimulation with IgG Fc, COS7 cells in 6-well plates were transfected with either pFUSE-mIgG1-Fc2, pFUSE-mIgG2A-Fc2, pFUSE-mIgG2B-Fc2, pFUSE-mIgG3-Fc2 (InvivoGen, San Diego, CA, USA), or an empty vector.

Techniques: Cell Culture, Immunolabeling, Control, Generated, Activation Assay, Inhibition